Tohoku University Technology: Visualization Probe for Protein Denaturation: T21-051
A method for chemically labeling denatured regions of proteins.
To visualize protein denaturation, conventional methods have developed chemical probes that bind to denatured regions of proteins, resulting in increased fluorescence intensity. However, the binding between traditional fluorescent probes and denatured protein regions was reversible. Therefore, in protein mixtures, it was difficult to link which protein's denaturation was causing the increase in fluorescence intensity. The fluorescent probe invented by Professor Shinichi Sato and his team at the Interdisciplinary Science Frontier Research Institute distinguishes itself from conventional denatured protein probes by forming a direct covalent bond with the denatured and aggregated sites of proteins. Additionally, it is a non-fluorescent molecule before the reaction and only emits fluorescence when it forms a covalent bond with aggregated proteins. Approximately 30 types of probes with varying detection sensitivities for denaturation have been developed so far. Furthermore, there are methods to concentrate the denatured and aggregated proteins, as well as their peptide fragments, allowing for mass spectrometry analysis of only the proteins that reacted with the probe.
- Company：Tohoku Techno Arch Co., Ltd.
- Price：Other
